Puesta a punto del análisis de oxitocina mediante inmunoensayo a partir de saliva de mujeres
Oxytocin (OXT) is known for its fundamental role in childbirth, lactation and maternal behavior thanks to its peripheral and central release from the hypothalamic-pituitary axis. In the Medical Imaging Laboratory of the Gregorio Marañón General Hospital, the aim is to analyze the concentration of OXT in saliva as part of a longitudinal neuroimaging study in women during pregnancy and postpartum. The present work has involved the development of an enzyme immunoassay (EIA) protocol for the detection of concentrations of the OXT hormone. To measure the reliability of the test, a test-retest analysis (calculation of intraclass correlation coefficient (ICC)) was carried out from 19 saliva samples of nulliparous women at two time points. The result obtained was moderate. Thanks to the present project, possible variables to be considered in the future are identified both in the pattern of acquisition of the samples and in the analysis methodology. From them, it is intended to continue optimizing the protocol to 2 reduce human intervention and time, improve the detection capacity and consequently increase the ICC.